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  • Title: Electrocatalytic activity of horseradish peroxidase/chitosan/carbon microsphere microbiocomposites to hydrogen peroxide.
    Author: Chen X, Li C, Liu Y, Du Z, Xu S, Li L, Zhang M, Wang T.
    Journal: Talanta; 2008 Oct 19; 77(1):37-41. PubMed ID: 18804595.
    Abstract:
    Colloidal carbon microspheres (CMS) are dispersed in chitosan (CHIT) solution to form an organic-inorganic hybrid with excellent micro-environment for the immobilization of biomolecules. A novel amperometric biosensor for the determination of hydrogen peroxide (H(2)O(2)) has been constructed by entrapping horseradish peroxidase (HRP) in as-synthesized CMS/CHIT hybrid. The modification of glassy carbon electrode is made by a simple solution-evaporation method. The electrochemical properties of the biosensor are characterized in electrochemical methods. The proposed biosensor shows high sensitive determination and fast response to H(2)O(2) at -0.15 V. The constructed HRP/CHIT/CMS/GC electrode also exhibits a fine linear correlation with H(2)O(2) concentration. The calculated value of the apparent Michaelis-Menten constant, 2.33 mM, suggests that the HRP in CMS/CHIT hybrid keeps its native bioactivity and has high affinity for H(2)O(2).
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