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  • Title: Opisthorchis viverrini: detection by polymerase chain reaction (PCR) in human stool samples.
    Author: Umesha KR, Kumar S, Parvathi A, Duenngai K, Sithithaworn P, Karunasagar I, Karunasagar I.
    Journal: Exp Parasitol; 2008 Dec; 120(4):353-6. PubMed ID: 18805413.
    Abstract:
    A polymerase chain reaction (PCR) assay was evaluated for detection of Opisthorchis viverrini eggs in the stool specimens of light and heavily infected individuals in Khon Kaen province of Thailand. A total of 75 fecal specimens were analyzed by PCR following DNA extraction. All the microscopically positive samples were positive by PCR, while 23 of 30 (76.6%) microscopically negative samples were also PCR positive. The sensitivity of the assay was 5 eggs/g of stool. This method is potentially useful in the diagnosis of human opisthorchiasis in endemic areas for treatment and in epidemiological investigations.
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