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  • Title: Resolution of (R,S)-(+/-)-glycidyl butyrate with immobilized Y-11 Trichosporon capitatum lipase in gelatin-containing microemulsion-based organogels.
    Author: Song X, Qi X, Qu Y.
    Journal: Colloids Surf B Biointerfaces; 2008 Nov 15; 67(1):127-31. PubMed ID: 18818057.
    Abstract:
    An extracellular lipase produced by the strain of Y-11 Trichosporon capitatum was purified to homogeneity by ammonium sulfate precipitation and four chromatographic steps. The purified lipase showed enhanced activity when it was immobilized in gelatin-containing microemulsion-based organogels. Furthermore, the resolution of (R,S)-(+/-)-glycidyl butyrate by this immobilized lipase gave a product of (S)-(-)-glycidol with approximately 98% ee (E=96). By using chiral HPLC separation, (S)-(-)-glycidol was obtained in enantiopure form. Scaled-up reaction in 2l shake flask was also performed and the repeated use of 15 times of immobilized Y-11 T. capitatum lipase resulted in little loss in its activity (4.8%).
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