These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: USPIO (Ferumoxtran-10)-enhanced MRI to visualize reticuloendothelial system cells in neonatal rats: feasibility and biodistribution study.
    Author: Azoulay R, Olivier P, Baud O, Verney C, Santus R, Robert P, Gressens P, Sebag G.
    Journal: J Magn Reson Imaging; 2008 Oct; 28(4):1046-52. PubMed ID: 18821607.
    Abstract:
    PURPOSE: To investigate whether USPIO-enhanced magnetic resonance imaging (MRI) detected reticuloendothelial system (RES) cells in newborn normal rats. MATERIALS AND METHODS: Newborn normal rats were imaged in vivo on a 1.5 T MR system, 2-96 hours after intraperitoneal Ferumoxtran-10 (n = 38) or saline injection (control group, n = 5). Signals from liver, spleen, and vertebral bone marrow were measured (T2-weighted Turbo Spin Echo) to describe the kinetics of enhancement. The pups were sacrificed and iron concentrations in plasma and peritoneal fluid were measured using atomic absorption spectrometry. Prussian blue-labeled cells density in liver, spleen, and vertebral bone marrow was assessed. RESULTS: Significant (P < 0.05) negative enhancement of the liver, spleen, and vertebral bone marrow was noted after Ferumoxtran-10 injection (2-96 hours for liver and spleen, 4-96 hours for bone marrow). Ferumoxtran-10 was absorbed from the peritoneum in the first 8 hours postinjection, entering the circulation with a plasma peak (8 hours); then Ferumoxtran-10 returned over the baseline in plasma (96 hours). Important intracellular iron deposition in liver and spleen was measured postinjection (3-96 hours, P < 0.05). Limited but significant intracellular iron deposition was noted in vertebral bone marrow postinjection (96 hours, P < 0.05), suggesting that Ferumoxtran-10 selectively labeled RES cells after 96 hours and produced nonspecific labeling at earlier timepoints. CONCLUSION: Ferumoxtran-10-enhanced MRI visualizes RES cells in vivo in newborn rats.
    [Abstract] [Full Text] [Related] [New Search]