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  • Title: Casein kinase I in bovine sperm: purification and characterization.
    Author: Chaudhry PS, Newcomer PA, Casillas ER.
    Journal: Biochem Biophys Res Commun; 1991 Aug 30; 179(1):592-8. PubMed ID: 1883382.
    Abstract:
    A highly purified preparation of sperm casein kinase I was obtained by sequential chromatography with phosphocellulose, gel filtration on sephacryl S-300, Affi-gel blue and DEAE-Cellulose. The chromatographic behavior and properties of the enzyme suggest that the sperm enzyme is similar to casein kinase I from other tissues. Antibodies against calf thymus casein kinase I cross-react with the sperm enzyme. A special feature of the sperm enzyme is that the activity is stimulated by spermine.
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