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  • Title: The rate of false positive sequence matches of peptides profiled by MALDI MS and identified by MS/MS.
    Author: Stoop MP, Lamers RJ, Burgers PC, Sillevis Smitt PA, Hintzen RQ, Luider TM.
    Journal: J Proteome Res; 2008 Nov; 7(11):4841-7. PubMed ID: 18837534.
    Abstract:
    In most MALDI peptide profiling cases, sequencing is required to identify peptides of interest, preferentially by using different mass spectrometry techniques. Using identical samples, we determined the number of false positive matches in sequence of peptide identification using different mass spectrometers. This paper demonstrates that the reliability of the identification phase greatly benefits from concerted MS-technologies and determines the influence of mass accuracy, signal-to-noise and statistical score on peptide identification.
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