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  • Title: MicroRNA profiling of megakaryocytes.
    Author: Garzon R.
    Journal: Methods Mol Biol; 2009; 496():293-8. PubMed ID: 18839118.
    Abstract:
    MiRNAs are non-coding RNAs of 19-25 nucleotides in length that regulate gene expression. Recent work suggests a role for miRNAs during hematopoiesis. Here, we described in detail how to successfully obtain highly pure megakaryocytes precursors from human CD34+ stem cells in culture to perform high-throughput miRNA profiling using a custom microarray platform.
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