These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Maintenance of functional activity of human pulmonary arteries after cryopreservation.
    Author: Ellis P, Müller-Schweinitzer E.
    Journal: Br J Pharmacol; 1991 Jun; 103(2):1377-80. PubMed ID: 1884097.
    Abstract:
    1. Human intrapulmonary arteries have been investigated in vitro in fresh tissue or after storage at -190 degrees C in foetal calf serum containing 1.8 M dimethyl sulphoxide. 2. After cryopreservation of the arteries, maximal contractile force was reduced to 76%. This was assessed by the responses (in g) to 10 nM of the thromboxane analogue, U 46619. 3. Constricting agonists such as noradrenaline, 5-hydroxytryptamine, histamine and U 46619 stimulated fresh and frozen/thawed arteries producing pD2 values similar to the respective values determined on fresh tissues. 4. Endothelium-independent relaxant responses of U 46619-precontracted arteries to prostacyclin (PGI2), aminophylline and papaverine were generally unchanged after storage. The same was true for relaxant response to the potassium channel activator P-1075 whereas the pD2 values for SDZ PCO 400, RP 49356 and cromakalim were somewhat diminished. 5. Nevertheless, a significant correlation was obtained when the apparent pD2 values for all agonists on fresh and frozen/thawed tissues were compared (P less than 0.001). 6. The evidence suggests that after cryopreservation of human intrapulmonary arteries at -190 degrees C, mechanisms of both contraction and relaxation are well-maintained.
    [Abstract] [Full Text] [Related] [New Search]