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  • Title: [The mechanism of apoptosis in human U87 glioma cells induced by miR-21 antisense oligonucleotide].
    Author: Shi L, Cheng Z, Zhang J, Li R, You Y, Fu Z.
    Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi; 2008 Oct; 25(5):497-501. PubMed ID: 18841558.
    Abstract:
    OBJECTIVE: To investigate the mechanism of U87 cell apoptosis induced by inhibiting miR-21 expression. METHODS: Antisense oligonucleotides of miR-21 were chemically synthesized and transfected into U87 cells. The apoptosis, proliferation, and invasion of the cells were evaluated. The relationship between miR-21 and PTEN or caspase was identified by bioinformatics and Western blot. RESULTS: Inhibiting miR-21 expression led to U87 cell growth suppression, apoptosis induction, invasion reduction, caspase-3 activity elevation and caspase-9 activation, but did not affect PTEN and caspase-8 expression. CONCLUSION: miR-21 may function as an antiapoptotic miRNA in U87 cells. Inhibiting miR-21 expression could induce U87 cell apoptosis via caspase-9 and 3 activation, but not PTEN activation.
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