These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [hNUDC promotes proliferation and differentiation of megakaryocytopoiesis on human CD341+ cells].
    Author: Chen YQ, Ge YC, Xu PL, Yang Y.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2008 Oct; 24(10):962-5. PubMed ID: 18845079.
    Abstract:
    AIM: aTo study the effect of human nuclear distribution C èhNUDCé on human megakaryocyte proliferation and differentiation from cord blood CD34(+) cells in vitro. METHODS: aHuman CD34(+) cells were isolated using the Dynal CD34 Progenitor Cell Selection System from umbilical cord blood. The CD34(+) cells were then cultured in serum free methylcellulose semi-solid media, the morphologic aspects and number of small, medium and large CFU-MK colonies were observed and scored on the day12 by microscopy analysis. The CD34(+) cells were cultured in serum free liquid media, cells were removed on day 10 and formation of CD41(+) in human megakaryocyte and its DNA polyploidization of nuclear were analyzed on a FACsort flowcytometer. RESULTS: ahNUDC supported the formation of small and medium CFU-MK colony in serum free semi-solid media. Furthermore, hNUDC induced a remarkable increase in expression of the megakaryocyte cell surface marker CD41(+) and stimulated the CD41(+) DNA polyploidization more effectively than TPO. CONCLUSION: hNUDC may play an important role in megakaryocyte proliferation and differentiation.
    [Abstract] [Full Text] [Related] [New Search]