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  • Title: Near full-length genome characterization of an HIV type 1 CRF25_cpx strain from Cameroon.
    Author: Luk KC, Holzmayer V, Ndembi N, Swanson P, Brennan CA, Ngansop C, Mbanya D, Kaptué L, Gürtler L, Devare SG, Hackett J.
    Journal: AIDS Res Hum Retroviruses; 2008 Oct; 24(10):1309-14. PubMed ID: 18851680.
    Abstract:
    Recombinant forms of HIV-1 contribute significantly to the ongoing epidemic. In the present study, we characterized the near full-length genome of one candidate HIV-1 CRF25_cpx strain originating in Cameroon, 06CM-BA-040. Viral RNA was extracted from plasma, and the genome was obtained using RT-PCR amplification to generate 10 overlapping fragments. Bootscanning, recombination breakpoint analysis, and phylogenetic trees confirmed that 06CM-BA-040 had a genomic structure consistent with two available CRF25_cpx reference sequences. The CRF25_cpx mosaic composition consisted of nine segments derived from subtypes A and G as well as unclassified (U) regions. Subtype G and CRF25_cpx clusters diverged from each other with long branch lengths but were distinct from other known subtypes with high bootstrap support (94%). The epidemiological significance of CRF25_cpx strains is unknown; however, the availability of additional genomic sequences will improve our understanding of the overall genetic diversity within this recombinant form of HIV-1.
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