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  • Title: Functionalization of multi-walled carbon nanotubes and their application for selective isolation of acidic proteins.
    Author: Du Z, Yu YL, Wang JH.
    Journal: Macromol Biosci; 2009 Jan 09; 9(1):55-62. PubMed ID: 18855949.
    Abstract:
    A micro-column packed with PDDA-wrapped MWNTs in sequential injection system facilitates selective sorption of acidic protein species. Proteins adsorbed onto the PDDA-MWNT composites are afterwards collected by elution with a citrate buffer as stripping reagent. With a sample loading volume of 2.0 mL and an eluent volume of 200 microL, a retention efficiency of 100% and a recovery of 90% are achieved for BSA in the range 6-120 microg, resulting in an enrichment factor of 14. A sampling frequency of 15 h(-1) is achieved, along with a precision of 4.5% at 25 microg x mL(-1) BSA. The practical applicability of this system is demonstrated by processing human whole blood for successive isolation of acidic proteins.
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