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  • Title: Complement C5a receptor assay for high throughput screening.
    Author: Harris SR, Garlick RK, Miller JJ, Harney HN, Monroe PJ.
    Journal: J Recept Res; 1991; 11(1-4):115-28. PubMed ID: 1886076.
    Abstract:
    The complement C5a receptor on U937 cells, a human histiocytic lymphoma cell line, stimulated with dibutyryl-cAMP have been stabilized for at least 3 months at a dilute, ready to use concentration. [125I]-Bolton Hunter labeled C5a, (recombinant, human) has been prepared by reverse phase HPLC to 2200 Ci/mmol. Using a filtration binding assay the Kd from receptor saturation analysis is 10-40 pM and there are 50,000-100,000 receptor sites per cell. These reagents have permitted the development of a reliable, reproducible and convenient drug screening assay, in kit format, for compounds acting at the C5a receptor.
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