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  • Title: [Expression of human lymphotoxin gene in CHO-DHFR- cells].
    Author: Liu JT, Li CB, Ni TH, Zhou YM, Zhao SY.
    Journal: Yi Chuan Xue Bao; 1991; 18(3):208-13. PubMed ID: 1888532.
    Abstract:
    A recombinant plasmid p91-HuLT was constructed with a human lymphotoxin (HuLT) gene 2.4 kb EcoRI fragment and a mammalian cell expression vector plasmid p91023. The HuLT EcoRI DNA fragment covers entire coding sequence and some 3(1) non-translated region sequence of the gene, p91-HuLT DNA was used to transfect Chinese hamster cell line CHO-DHFR- cells by using calcium phosphate-DNA coprecipitation method, and DHFR+ transfectants were obtained. RNA dot blot hybridization analysis showed that the HuLT mRNA was produced by p91-HuLT in transfectants. The results of MTT dye reduction assay indicated that the DHFR+ cell lines we obtained constitutively synthesize and secrete the HuLT with the cytotoxic activity of at least 200 units per mL of medium.
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