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  • Title: The impact of protein exclusion on the purity performance of ion exchange resins.
    Author: Zeid J, Harinarayan C, van Reis R.
    Journal: Biotechnol Bioeng; 2009 Feb 15; 102(3):971-6. PubMed ID: 18942152.
    Abstract:
    Dynamic binding capacity (DBC) decreases with increasing conductivity in the equilibrium regime for ion exchange chromatography. An exclusion regime has been demonstrated in ion exchange resins where DBC increases with increasing conductivity and decreasing protein charge. The purpose of this work was to examine the impact of the exclusion regime on impurity removal. Resin performance was evaluated based on dynamic binding capacities and purity within the exclusion and equilibrium regimes. The results revealed that Chinese hamster ovary proteins (CHOP), a major impurity, exhibit similar exclusion trends as the MAb proteins. The results further the understanding of the exclusion regime and its impact on product purity, a critical area for IEX development and optimization.
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