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  • Title: Detection of the nuclear poly(ADP-ribose)-metabolizing enzymes and activities in response to DNA damage.
    Author: Amé JC, Hakmé A, Quenet D, Fouquerel E, Dantzer F, Schreiber V.
    Journal: Methods Mol Biol; 2009; 464():267-83. PubMed ID: 18951190.
    Abstract:
    Poly(ADP-ribosyl)ation is a posttranslational modification of proteins in higher eukaryotes mediated by poly(ADP-ribose) polymerases (PARPs) that is involved in many physiological processes such as DNA repair, transcription, cell division, and cell death. Biochemical studies together with PARP-1- or PARP-2-deficient cellular and animal models have revealed the redundant but also complementary functions of the two enzymes in the surveillance and maintenance of genome integrity. Poly(ADP-ribose) is degraded by the endo- and exo-glycosidase activities of poly(ADP-ribose) glycohydrolase (PARG). In this chapter, biochemical and immunofluorescence methods are described for detecting and assaying PARPs and PARG.
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