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  • Title: Spectrophotometric determination of saccharin in different materials by a solvent extraction method using Nile Blue as reagent.
    Author: Cordoba MH, Garcia IL, Sanchez-Pedreño C.
    Journal: Talanta; 1985 Apr; 32(4):325-7. PubMed ID: 18963851.
    Abstract:
    In a slightly acidic medium (sodium acetate-acetic acid buffer, pH 4) saccharin and Nile Blue form an ion-association compound which is extractable into methyl isobutyl ketone and allows the spectrophotometric determination of saccharin. At 630 nm Beer's law is obeyed over the saccharin concentration range 0.1-3.5 microg/ml in the aqueous phase and the apparent molar absorptivity is 5.8 x 10(4) l.mole(-1).cm(-1). The method shows good selectivity and can be applied to the determination of saccharin in artificial sweeteners, soft drinks and toothpastes.
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