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  • Title: On-line extraction-spectrophotometric determination of neostigmine in pharmaceuticals using double membrane phase separator and monovalent dyestuff.
    Author: Sakai T, Liu X, Maeda Y.
    Journal: Talanta; 1999 Aug 09; 49(5):997-1001. PubMed ID: 18967676.
    Abstract:
    A simple, sensitive and rapid spectrophotometric method for the determination of neostigmine by flow injection analysis (FIA) coupled with an ion associate extraction has been developed. The three-line manifold was assembled. Neostigmine(200 mul) was injected into a distilled water stream and the pH was adjusted to 10 with a borate-phosphate buffer solution. Then, the stream was mixed with the ion-pairing tetrabromophenolphthalein ethylester (TBPEH)-1,2-dichloroethane solution. After phase separation with a double membrane phase separator, absorbance was measured at 610 nm. A linear calibration graph was obtained between 1x10(-7) mol l(-1) and 5x10(-7) mol l(-1) of neostigmine. Up to 48 samples h(-1) could be processed with a relative standard deviation (R.S.D.) of 0.5% (n=5) for 4x10(-7) mol l(-1) neostigmine. The proposed system was applied to the simple, reproducible and rapid determination of neostigmine in commercial pharmaceuticals.
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