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  • Title: Voltammetric microanalysis of DNA adducts with osmium tetroxide,2,2'-bipyridine using a pyrolytic graphite electrode.
    Author: Fojta M, Havran L, Kizek R, Billová S.
    Journal: Talanta; 2002 Apr 01; 56(5):867-74. PubMed ID: 18968565.
    Abstract:
    DNA and synthetic polynucleotides modified with a complex of osmium tetroxide with 2,2'-bipyridine (Os,bipy) produce specific voltammetric signals at pyrolytic graphite electrodes. Based on a sufficient potential separation between the peaks of Os,bipy-modified DNA (DNA-Os,bipy) and of free Os,bipy, and using an adsorptive transfer stripping voltammetric procedure involving extraction of free Os,bipy from the electrode by chloroform, DNA-Os,bipy can be determined in an excess of the free reagent. Under certain conditions, 140 pg of DNA-Os,bipy can be detected after a 5 min accumulation period. This analysis displays a more favorable sensitivity and a better selectivity for DNA structure than oxidation of DNA guanine moieties, and offers detection of osmium DNA markers at carbon electrodes.
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