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  • Title: Quantification of methylated DNA by HeavyMethyl duplex PCR.
    Author: Distler J.
    Journal: Methods Mol Biol; 2009; 507():339-46. PubMed ID: 18987825.
    Abstract:
    The HeavyMethyl (HM) assay is a real-time PCR assay suitable for the qualitative and quantitative DNA methylation analysis of fresh, frozen, or formalin-fixed paraffin-embedded tissues and remote samples, such as serum, plasma, and urine. The HM uses a methylation-specific oligonucleotide blocker and a methylation-specific probe to achieve methylation-specific amplification and detection. A protocol for a duplex real-time PCR for the analysis of the methylation status of the GSTP1 exon1 in prostate tissue samples is presented. This chapter describes the preparation and analysis of a combined HM GSTP1 Exon1 and GSTP1 reference assay.
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