These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Analysis of the antigenicity of the expression product of fusion antigen epitope genes of FMDV].
    Author: Wang DM, Shen WT, Li XY, Zhou P.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2008 Nov; 24(11):1048-50. PubMed ID: 18992188.
    Abstract:
    AIM: From the different recombinants of FMDV antigen epitope, the highest antigenicity one was selected, and that provided research foundation for making researches on FMD plant-based vaccines which could effectively induce cell immune response and be characterized by high eurytopicity. METHODS: The single-chain DNA of 5 T-cell epitope genes and 2 B-cell epitope genes were synthesized. By gene splicing by overlap extension technique, 5 T-cell epitope genes were fused together into T and 2 B-cell epitope genes were fused together into B. With Ava III and Pst I which produced uniform stickiness terminal, T and B were fused into recombinats in which T and B existed in different position, namely 5'-T-B-T-3', 5'-T-T-B-3' and 5'-B-T-T-3'. With potato X virus expression vector (PVX) every antigen gene (T, B, T-B-T, T-T-B, B-T-T and VP1) was expected to be expressed in leaves of tobacco. RT-PCR analysis and conformed whether all of the antigen genes were transcribed. Indirect ELISA Western Dotblot conformed analysed the antigeni-city of these products. RESULTS: Every antigen genes were expressed successfully. The different products had different antigenicity, and 5'-T-B-T -3' was highest, 5'-B-T-T-3' was the second, and 5'-T-T-B-3' was lowest, but 5'-T-T-B-3' was higher than VP1, T or B. CONCLUSION: It is possible that gene engineering vaccine against FMDV with high eurytopicity and the induction of cell-immune response could be obtained by the different fusion ways with T and B cell epitopes existing in different position of recombinats, and that could have effect on immune activity of vaccines.
    [Abstract] [Full Text] [Related] [New Search]