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  • Title: Characterization of HCV quasispecies in the hypervariable region 1 (HVR1) by in vitro translation and mass spectrometry.
    Author: Yea C, Ayers M, Tellier R.
    Journal: Methods Mol Biol; 2009; 510():73-81. PubMed ID: 19009254.
    Abstract:
    HCV infection provides a classic example of the phenomenon of quasispecies. Because several lines of investigation support the contribution of quasispecies to HCV's capacity to maintain a persistent infection, adequate characterization of the quasispecies is important. The hypervariable region 1 (HVR1) of the E2 glycoprotein has been particularly well studied in this regard. We present here a rapid method for characterizing the HVR1 quasispecies, based on in vitro coupled transcription/translation of the amplicons, followed by mass spectrometry of the resulting peptide mix.
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