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  • Title: Differences in high-energy phosphate catabolism between the rat and the dog in a heart preservation model.
    Author: Minten J, Segel LD, Van Belle H, Wynants J, Flameng W.
    Journal: J Heart Lung Transplant; 1991; 10(1 Pt 1):71-8. PubMed ID: 1901025.
    Abstract:
    Concentrations of ATP and creatine phosphate were measured in rat and dog hearts preserved either by cold storage (procedure A) or by continuous hypothermic perfusion (procedure B). In procedure A (3 dogs, 4 rats) the hearts were normothermically excised without cardioplegia and were stored in 0.9% NaCl at 0.5 degrees C; in procedure B (6 dogs, 21 rats) hypothermic cardioplegic arrest was performed, and then the hearts were retrogradely perfused through the aorta for 24 hours with use of an oxygenated Bretschneider cardioplegic solution at 2 degrees to 4 degrees C. Whole rat hearts were frozen using Wollenberger clamps at desired times during the preservation period; transmural needle biopsy specimens were sampled from dog hearts. In control nonpreserved hearts, the ATP and creatine phosphate were as follows (mean +/- SD): 26.7 +/- 4.1 and 27.1 +/- 10.3 mumol/gm dry weight, respectively (dog hearts), and 23.1 +/- 2.1 and 34.2 +/- 12.1 mumol/gm dry weight, respectively (rat hearts). With procedure A, ATP decreased by 36% in dog hearts and by 64% in rat hearts during the first hour of storage. By 24 hours, only 6% of the ATP remained in the dog hearts and 1% in the rat hearts. Creatine phosphate decreased by 85% (dog hearts) and by 93% (rat hearts) during the first hour of storage. The ATP and creatine phosphate values observed in rat hearts after 1 hour of procedure A preservation were significantly lower than in dog hearts (p less than 0.05). With procedure B, cardioplegic arrest by itself did not alter high-energy phosphate concentrations in dog or rat hearts.(ABSTRACT TRUNCATED AT 250 WORDS)
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