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  • Title: High sensitivity detection of 16s rRNA using peptide nucleic acid probes and a surface plasmon resonance biosensor.
    Author: Joung HA, Lee NR, Lee SK, Ahn J, Shin YB, Choi HS, Lee CS, Kim S, Kim MG.
    Journal: Anal Chim Acta; 2008 Dec 23; 630(2):168-73. PubMed ID: 19012828.
    Abstract:
    A signal enhancing method allowing highly sensitive detection of E. coli 16s rRNA was developed using peptide nucleic acid (PNA) as a capture probe and a surface plasmon resonance (SPR) sensor as a detector. 16s rRNA has been used as a genetic marker for identification of organisms, and can be analyzed directly without PCR amplification due to the relatively high number of copies. PNA has a neutral backbone structure, therefore hybridization with 16s rRNA results in the ionic condition being changed from neutral to negative. A cationic Au nanoparticle was synthesized and used for signal amplification by ionic interaction with 16s rRNA hybridized on the PNA probe-immobilized SPR sensor chip. This method resulted in a detection limit of E. coli rRNA of 58.2+/-1.37 pg mL(-1). Using this analytical method, Staphylococcus aureus was detected without purification of rRNA.
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