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  • Title: Actin depolymerization in the cyclic AMP-stimulated toad bladder epithelial cell, determined by the DNAse method.
    Author: Hays RM, Lindberg U.
    Journal: FEBS Lett; 1991 Mar 25; 280(2):397-9. PubMed ID: 1901549.
    Abstract:
    Previous studies with the rhodamine phalloidin binding assay have shown that antidiuretic hormone and 8-Br-cAMP rapidly depolymerize F-actin in toad bladder epithelial cells. We have extended these studies with the DNAse inhibition assay and have found that in isolated epithelial cell suspensions, G-actin increases from 37 to 56% of total actin following 8-br-cAMP stimulation. The G-actin concentration in the epithelial cell greatly exceeds its critical concentration, indicating the requirement for a G-actin sequestering protein or proteins in this system.
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