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  • Title: [Determination of monosaccharides in honey and polysaccharide hydrolyzate of functional food by high performance anion exchange chromatography].
    Author: Zou XL, Jiang S, Zheng B, Zeng HY, Yan CL, Xie Q.
    Journal: Sichuan Da Xue Xue Bao Yi Xue Ban; 2008 Sep; 39(5):836-8. PubMed ID: 19024327.
    Abstract:
    OBJECTIVE: To develop a method for analysis of eight kinds of monosaccharides using high performance anion exchange chromatography. METHODS: Amino PAC PA-10 (2 x 250 mm) column with a pulse amperometric detector with an Au working electrode and an Ag/AgCl reference electrode was applied to determine the monosaccharides. RESULTS: The linear range was 0.1-5.0 microg/mL and the detection limits were 2-26 microg/L. The relative standard derivation for retain time and area of standard solution were 0.34%-7.42% and 0.02%-2.16% respectively. The satisfactory results were obtained for analysis of honey and polysaccharide hydrolyzate of functional food with the proposed method. The average recoveries were 81.4%-122.0%. The method was sensitive and with high precision. CONCLUSION: This method was available for determination of monosaccharides in honey and polysaccharide hydrolyzate of functional food.
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