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  • Title: [Detection of HBV DNA among HBsAg negative children after prevention for mother-to-infant transmission].
    Author: Zhang S, Bian T, Wang F, Shen LP, Yan HX, Zhang Y.
    Journal: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 2008 Jun; 22(3):214-5. PubMed ID: 19031707.
    Abstract:
    OBJECTIVE: Detection HBV DNA among HBsAg negative children who have been vaccined at birth, in order to improve the evaluation of the indicator for HBV DNA infection. METHODS: Selection HBsAg negative children who have been vaccined at birth and then detection HBV DNA from sera using QIAamp Viral DNA Mini Kit, HBV DNA s region was obtained by nested PCR and sequencing. RESULTS: 12 of the 140 children were HBV DNA detected were positive and the infectious rate was 8.6% . No mutant of the 12 HBV DNA in "a" determinant. CONCLUSION: To evaluate the effection of the prevention of HBV mother-to-child transmission, the standard method should be established. The detection of HBV DNA should be included in the future.
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