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  • Title: Comparative transcriptome analysis between original and evolved recombinant lactose-consuming Saccharomyces cerevisiae strains.
    Author: Guimarães PM, Le Berre V, Sokol S, François J, Teixeira JA, Domingues L.
    Journal: Biotechnol J; 2008 Dec; 3(12):1591-7. PubMed ID: 19039778.
    Abstract:
    The engineering of Saccharomyces cerevisiae strains for lactose utilization has been attempted with the intent of developing high productivity processes for alcoholic fermentation of cheese whey. A recombinant S. cerevisiae flocculent strain that efficiently ferments lactose to ethanol was previously obtained by evolutionary engineering of an original recombinant that displayed poor lactose fermentation performance. We compared the transcriptomes of the original and the evolved recombinant strains growing in lactose, using cDNA microarrays. Microarray data revealed 173 genes whose expression levels differed more than 1.5-fold. About half of these genes were related to RNA-mediated transposition. We also found genes involved in DNA repair and recombination mechanisms, response to stress, chromatin remodeling, cell cycle control, mitosis regulation, glycolysis and alcoholic fermentation. These transcriptomic data are in agreement with some of the previously identified physiological and molecular differences between the recombinants, and point to further hypotheses to explain those differences.
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