These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Myo-inositol facilitators IolT1 and IolT2 enhance D-mannitol formation from D-fructose in Corynebacterium glutamicum.
    Author: Bäumchen C, Krings E, Bringer S, Eggeling L, Sahm H.
    Journal: FEMS Microbiol Lett; 2009 Jan; 290(2):227-35. PubMed ID: 19054080.
    Abstract:
    Reduction of D-fructose to D-mannitol by whole-cell biotransformation with recombinant resting cells of Corynebacterium glutamicum ATCC13032 requires the coexpression of mdh and fdh, which encode mannitol and formate dehydrogenases, respectively. However, d-mannitol formation is limited by the uptake of d-fructose in its unphosphorylated form, because additional expression of the sugar facilitator from Zymomonas mobilis resulted in a significantly increased productivity. Here we identified similarities of the myo-inositol transporters IolT1 and IolT2 of C. glutamicum to the sugar facilitator of Z. mobilis. The myo-inositol transporter genes were both individually overexpressed and deleted in recombinants expressing mdh and fdh. Biotransformation experiments showed that the presence and absence, respectively, of IolT1 and IolT2 significantly influenced D-mannitol formation, indicating a D-fructose transport capability of these transporters. For further evidence, a C. glutamicum Delta ptsF mutant unable to grow with D-fructose was complemented with a heterologous fructokinase gene. This resulted in restoration of growth with D-fructose. Using overexpressed iolT1, mdh and fdh, D-mannitol formation obtained with C. glutamicum was 34.2 g L(-1), as opposed to 16 g L(-1) formed by the strain overexpressing only mdh and fdh, showing the suitability of myo-inositol transporters for D-fructose uptake to obtain D-mannitol formation by whole-cell biotransformation with C. glutamicum.
    [Abstract] [Full Text] [Related] [New Search]