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  • Title: Determination of the branch location of extra N-acetyllactosamine units in sialo N-linked tetraantennary oligosaccharides.
    Author: Hokke CH, Kamerling JP, van Dedem GW, Vliegenthart JF.
    Journal: FEBS Lett; 1991 Jul 29; 286(1-2):18-24. PubMed ID: 1907570.
    Abstract:
    An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-beta-galactosidase, followed by treatment with N-acetyl-beta-glucosaminidase, yielding products which could be analysed by 1H-NMR spectroscopy, thereby giving conclusive data about the location of the extra units in the intact structures.
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