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  • Title: Inhibition of pertussis toxin catalyzed ADP-ribosylation of G-proteins by membrane depolarization in rat brain synaptoneurosomes.
    Author: Cohen-Armon M, Sokolovsky M.
    Journal: Neurosci Lett; 1991 May 13; 126(1):87-90. PubMed ID: 1907726.
    Abstract:
    Rat brainstem synaptoneurosomes at resting and depolarization potentials were subjected to ADP-ribosylation in the presence of pertussis toxin (PTX). Subsequent [32P]ADP-ribosylation of synaptoneurosomal membranes revealed labeling of a 39-kDa protein band which reacted with antibodies to the alpha-subunit of G-proteins, mainly Go. ADP-ribosylation of the G-proteins was completely achieved in synaptoneurosomes at resting potential ( [K+] = 4.7 mM). In the depolarized synaptoneurosomes, however, the higher the membrane potential the lower the extent of ADP-ribosylation achieved (46% and 11% in K+ concentrations of 50 and 100 mM, respectively). A similar effect of membrane depolarization on PTX-catalyzed ADP-ribosylation was expressed in the functional coupling between G-protein activation and changes induced in the muscarinic receptor affinity. These findings may indicate a depolarization-induced inhibition of PTX-catalyzed ADP-ribosylation of G-proteins.
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