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  • Title: Purification and characterization of a cell wall hydrolase encoded by the cwlA gene of Bacillus subtilis.
    Author: Kuroda A, Imazeki M, Sekiguchi J.
    Journal: FEMS Microbiol Lett; 1991 Jun 01; 65(1):9-13. PubMed ID: 1908396.
    Abstract:
    A cell wall hydrolase of Bacillus subtilis was prepared from Escherichia coli cells harboring a plasmid containing the B. subtilis cwlA gene and purified by hydroxyapatite column chromatography and HPLC through TSK-gel G3000SWXL. In contrast to the molecular mass of 29,919 Da deduced from its nucleotide sequence, the purified CWLA is a 23 kDa protein. Characterization of the specific substrate bond cleaved by CWLA indicated the enzyme is an N-acetylmuramyl-L-alanine amidase. A 32-kDa precursor protein was detected on zymography of a crude cell homogenate. Some of the enzymatic properties of CWLA are also described.
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