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  • Title: Expression of exogenous mRNA in Xenopus laevis embryos for the study of cell cycle regulation.
    Author: Sible JC, Wroble BN.
    Journal: Methods Mol Biol; 2009; 518():1-15. PubMed ID: 19085142.
    Abstract:
    The microinjection of mRNA that is transcribed and capped in vitro into fertilized eggs and embryos of Xenopus laevis provides a powerful means for discovering the function of proteins during early development. Proteins may be overexpressed for a gain-of-function effect or exogenous protein function may be compromised by the microinjection of mRNA encoding "dominant-negative" proteins. This methodology is particularly suited for the investigation of the regulation of the cell cycle, checkpoints, and apoptosis in early development.
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