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  • Title: [Application of orthogonal design in tissue culture of Dioscorea opposite].
    Author: Xue JP, Zhao XQ, Zhang AM, Huang YQ.
    Journal: Zhongguo Zhong Yao Za Zhi; 2008 Aug; 33(16):1952-5. PubMed ID: 19086626.
    Abstract:
    OBJECTIVE: To study the effects of different factors on buds and microtuber. These factors included plant growth substances and sucrose. METHOD: stems were selected as explants. The effects of three kinds of factors were studied by orthogonal design method including sucrose, 6-BA, NAA on the buds and microtuber induction. The data were analyzed with range analysis and vadance analysis. RESULT AND CONDUSION: The optimal media to induce many buds from stems were MS + 6-BA 1 mg x L(-1) + NAA 1 mg x L(-1) + sucrose 3%, the effect of the three factors was in sequence of sucrose >6-BA > NAA. The optimal media to induce microtuber from stems were MS+6-BA 1.5 mg x V1 +NAA 1.5 mg x L(-1) + sucrose 5%, the effect of the three factors was in sequence of sucrose >6-BA > NAA.
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