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  • Title: Separation of glutaraldehyde and some of its aldol condensation products by hydroxyl-aldehyde group affinity chromatography.
    Author: Holmquist L, Lewin M.
    Journal: J Biochem Biophys Methods; 1991; 22(4):321-9. PubMed ID: 1908872.
    Abstract:
    Glutaraldehyde exists in aqueous solution in equilibrium with monomers and polymers of cyclic and open-chain hemialdals and hydrates. At alkaline pH oligomeric and polymeric alpha,beta-unsaturated aldehyde derivatives are formed from primarily produced aldol condensation products. This communication reports a method for separation of such aldol condensates by means of a new high performance liquid chromatography technique based on the affinity of aldehyde groups for hydroxyl groups of a hydroxylated polyether matrix (Bio-Gel SEC-10). Five peaks corresponding to different aldol condensates of glutaraldehyde were obtained from the affinity column. They have been distinguished by 1H-NMR and UV spectroscopy. Kinetic measurements yielded formation rates for the different aldol condensates.
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