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  • Title: Lysophosphatidylcholine induces endothelial cell injury by nitric oxide production through oxidative stress.
    Author: Kim EA, Kim JA, Park MH, Jung SC, Suh SH, Pang MG, Kim YJ.
    Journal: J Matern Fetal Neonatal Med; 2009 Apr; 22(4):325-31. PubMed ID: 19089771.
    Abstract:
    OBJECTIVE: To determine whether lysophosphatidylcholine (LPC) induces endothelial cell injury by altering the production of nitric oxide (NO) and thereby increasing reactive oxygen species (ROS). METHODS: Human umbilical vein endothelial cells (HUVECs) were cultured and exposed to LPC, LPC with N(G)-nitro-l-arginine methyl ester (L-NAME), LPC with antioxidants. LPC-induced cell injury and viability were determined using LDH and Resazurin assays. The Mann-Whitney U test was used for statistical analysis. RESULTS: LPC induced HUVEC injury in a concentration-dependent manner. LPC induced the overproduction of NO and ROS in HUVECs and LPC-induced HUVEC injury is significantly inhibited by the eNOS inhibitor (L-NAME) and the antioxidants (p < 0.05). CONCLUSIONS: These findings suggest that LPC induces the overproduction of NO, which may increase the oxidative stress on endothelial cells and lead to endothelial cell injury.
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