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  • Title: Methanogenic pathways in Methanosphaera stadtmanae.
    Author: van de Wijngaard WM, Creemers J, Vogels GD, van der Drift C.
    Journal: FEMS Microbiol Lett; 1991 May 15; 64(2-3):207-11. PubMed ID: 1909277.
    Abstract:
    Methanosphaera stadtmanae reduces methanol to CH4 in a similar way as Methanosarcina barkeri. Low activities of 5,10-methylenetetrahydromethanopterin dehydrogenase (MTDH) and reductase (MTR) were found. From studies on formaldehyde oxidation and reduction it was concluded that most likely the inability to reduce CO2 to CH4 was due to the lack of an active or the presence of an inactive CO2 reductase system and methyltetrahydromethanopterin (methyl-H4MPT): coenzyme M methyltransferase. Methanofuran was not detected, while the presence of a pterin, analogous to H4MPT, could be substantiated from its degradation products in boiled extracts.
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