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  • Title: Determination of serum apolipoprotein B by competitive enzyme-linked immunoassay.
    Author: Reisinger H, Strobl W, Hubrich B, Widhalm K.
    Journal: J Inherit Metab Dis; 1991; 14(2):247-52. PubMed ID: 1909404.
    Abstract:
    An enzyme-linked immunoassay was used to determine apolipoprotein B (apoB) in serum samples from 104 healthy adults aged 18-26 years. The method was compared to radial immunodiffusion (RID). Mean apoB values were 103 +/- 30 mg/dl and 105 +/- 31 mg/dl respectively. The correlation coefficient for apoB between the two methods was r = 0.70, p less than 0.001; the correlation coefficient between LDL-cholesterol and apoB measured by the ELISA was r = 0.73, p less than 0.001. This ELISA technique seems to be an appropriate method for measuring apoB in plasma, in the clinical laboratory and for detecting individuals with hyperapobetalipoproteinaemia.
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