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  • Title: Generation of hepatocytes from human embryonic stem cells.
    Author: Safinia N, Minger SL.
    Journal: Methods Mol Biol; 2009; 481():169-80. PubMed ID: 19096802.
    Abstract:
    Use of human hepatocytes for therapeutic and drug discovery applications is hampered by limited tissue source and the inability of hepatocytes to proliferate and maintain function long-term in vitro. Human embryonic stem (hES) cells are immortal and pluripotent and may provide a cell source for functional human hepatocytes (1) Here we have outlined some of the protocols currently in use for the generation of hepatocytes from hES cells.
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