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  • Title: Chapter 12: Nanoscale biological fluorescence imaging: breaking the diffraction barrier.
    Author: Gould TJ, Hess ST.
    Journal: Methods Cell Biol; 2008; 89():329-58. PubMed ID: 19118681.
    Abstract:
    Biological imaging has been limited by the finite resolution of light microscopy. Recent developments in ultra-high-resolution microscopy methods, many of which are based on fluorescence, are breaking the diffraction barrier; it is becoming possible to image intracellular protein distributions with resolution of tens of nanometers or better. Fluorescence photoactivation localization microscopy (FPALM) is an example of such an ultra-high-resolution method which can image living or fixed cells with demonstrated lateral resolution of better than 20 nm. A detailed description of the methods involved in FPALM imaging of biological samples is presented here, accompanied by comparison with existing methods from the literature.
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