These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Carbohydrate binding specificity of recombinant human macrophage beta-glucan receptor dectin-1.
    Author: Ujita M, Nagayama H, Kanie S, Koike S, Ikeyama Y, Ozaki T, Okumura H.
    Journal: Biosci Biotechnol Biochem; 2009 Jan; 73(1):237-40. PubMed ID: 19129647.
    Abstract:
    Human macrophage dectin-1, a type II transmembrane beta-glucan receptor, was expressed as a fusion protein with an N-terminal hexahistidine tag and glutathione S-transferase in an Escherichia coli cell-free translation system, and assayed for binding specificity. Recombinant dectin-1 specifically bound to some beta-glucans, but not to other carbohydrates. The beta-glucan binding of recombinant dectin-1 was inhibited by laminarin, a soluble beta-glucan, and by laminarioligosaccharides, but not by other carbohydrates. These results suggest that recombinant human dectin-1 can be used as a useful probe in identifying ligands in humans and tonic foods due to its strict binding specificity.
    [Abstract] [Full Text] [Related] [New Search]