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  • Title: Transport and storage of sputum specimen by using cetylpyridinium chloride for isolation of mycobacteria.
    Author: Pal N, Sharma B, Malhotra B, Rishi S.
    Journal: Indian J Pathol Microbiol; 2009; 52(1):59-61. PubMed ID: 19136783.
    Abstract:
    Of the 191 sputum specimens that were collected from pulmonary tuberculosis patients, 78.65% (140/178) specimens were culture positive when processed within 48 h by the NaOH method. The culture positivity in the same specimen that were stored with cetylpyridinium chloride (CPC) and processed after 7-8 days was 70.22% (125/178), whereas those stored without CPC and processed by the NaOH method was 46.62% (83/178). The difference in number of positive cultures obtained before storage and after storage (without CPC) was statistically significant (P = 0.001). Culture positivity by the CPC method was comparable with that of NaOH method before storage and the difference was not statistically significant (P = 0.35).
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