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  • Title: Controlling the translocation of single-stranded DNA through alpha-hemolysin ion channels using viscosity.
    Author: Kawano R, Schibel AE, Cauley C, White HS.
    Journal: Langmuir; 2009 Jan 20; 25(2):1233-7. PubMed ID: 19138164.
    Abstract:
    Translocation of single-stranded DNA through alpha-hemolysin (alpha-HL) channels is investigated in glycerol/water mixtures containing 1 M KCl. Experiments using glass nanopore membranes as the lipid bilayer support demonstrate that the translocation velocities of poly(deoxyadenylic acid), poly(deoxycytidylic acid), and poly(deoxythymidylic acid) 50-mers are decreased by a factor of approximately 20 in a 63/37 (vol %) glycerol/water mixture, relative to aqueous solutions. The ion conductance of alpha-HL and the entry rate of the polynucleotides into the protein channel also decrease with increasing viscosity. Precise control of translocation parameters by adjusting viscosity provides a potential means to improve sequencing methods based on ion channel recordings.
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