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  • Title: Mitogen-activated protein kinase cascade in the signaling for polyamine biosynthesis in tobacco.
    Author: Jang EK, Min KH, Kim SH, Nam SH, Zhang S, Kim YC, Cho BH, Yang KY.
    Journal: Plant Cell Physiol; 2009 Mar; 50(3):658-64. PubMed ID: 19151070.
    Abstract:
    Expression of NtNEK2(DD), a constitutively active mutant of NtMEK2, activates endogenous salicylic acid-induced protein kinase (SIPK) and wounding-induced protein kinase (WIPK), and leads to several stress/defense responses in tobacco. In this study, we used ACP (annealing control primer)-based differential display reverse transcription-PCR to isolate the downstream effectors mediated by the NtMEK2-SIPK/WIPK cascade. The arginine decarboxylase gene (ADC), which is involved in plant putrescine biosynthesis, was one of nine differentially expressed genes. When compared with NtMEK2(KR) plants, NtMEK2(DD) transgenic plants exhibited a significant increase in ADC and ODC (ornithine decarboxylase) transcript levels, as well as in putrescine and its catabolite, gamma-aminobutyric acid, following SIPK/WIPK activation. Taken together, these results suggest that the NtMEK2-SIPK/WIPK cascade is involved in regulating polyamine synthesis, especially putrescine synthesis, through transcriptional regulation of the biosynthetic genes in tobacco.
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