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  • Title: Protective effect of peroxisome proliferator-activated receptor-gamma agonists on activated renal proximal tubular epithelial cells in IgA nephropathy.
    Author: Xiao J, Leung JC, Chan LY, Guo H, Lai KN.
    Journal: Nephrol Dial Transplant; 2009 Jul; 24(7):2067-77. PubMed ID: 19155534.
    Abstract:
    BACKGROUND: We have previously demonstrated a glomerulo-tubular 'crosstalk' operating in the pathogenesis of tubulointerstitial injury in IgA nephropathy (IgAN). The present study aims to explore any possible beneficial effect of a peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist in alleviating the tubulointerstitial inflammation in IgAN. METHODS: Human proximal tubular epithelial cells (PTEC) were pre-treated with increasing concentration of a PPAR-gamma agonist rosiglitazone or troglitazone (0-5 microM) followed by further incubation with the conditioned medium (IgA-HMC) collected from human mesangial cells (HMC) incubated with polymeric IgA isolated from IgAN patients. Gene expression of interleukin-6 (IL-6) and angiotensin II type 1 receptor (ATR1) was detected by reverse transcription-polymerase chain reaction (RT-PCR); protein expression of IL-6 and ATR1 was determined by ELISA and western blot, respectively. The mitogen-activated protein kinase extracellular signal-related kinase 1/2 (ERK1/2) activation was examined by western blot. RESULTS: An IgA-HMC conditioned medium prepared from IgAN patients increased gene expression and protein synthesis of IL-6 and ATR1 in PTEC when compared with a conditioned medium prepared from healthy controls. The upregulated gene expression and protein synthesis of IL-6 and ATR1 in PTEC induced by the IgA-HMC conditioned medium were readily attenuated following pre-treatment with a PPAR-gamma agonist, thiazolidinedione (TZD). The ATR1-downregulating effect exerted by the PPAR-gamma agonist occurred through the inhibition of ERK1/2 activation. The PPAR-gamma antagonist, GW9662, significantly attenuated the inhibitory action of rosiglitazone on the increased synthesis of IL-6 and ATR1 protein. CONCLUSION: Our current findings suggest that the PPAR-gamma agonist attenuates excessive inflammatory response in activated PTEC in IgAN through suppressing ATR1 expression. This ATR1-downregulating effect is likely through the inhibition of ERK1/2 activation and is found to be PPAR-gamma dependent. TZDs may possibly be new therapeutic additives to established treatment regime for renin-angiotensin system (RAS) blockade in IgAN.
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