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  • Title: A simple and sensitive HPLC-ESI-MS/MS method for the determination of andrographolide in human plasma.
    Author: Xu L, Xiao DW, Lou S, Zou JJ, Zhu YB, Fan HW, Wang GJ.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2009 Feb 15; 877(5-6):502-6. PubMed ID: 19158000.
    Abstract:
    A liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method for the determination of andrographolide in human plasma was established. Dehydroandrographolide was used as the internal standard (I.S.). The plasma samples were deproteinized with methanol and separated on a Hanbon C(18) column with a mobile phase of methanol-water (70:30, v/v). HPLC-ESI-MS/MS was performed in the selected ion monitoring (SIM) mode using target ions at [M-H(2)O-H](-), m/z 331.1 for andrographolide and [M-H](-), m/z 331.1 for the I.S. Calibration curve was linear over the range of 1.0-150.0ng/mL. The chromatographic separation was achieved in less than 6.5min. The lower limits of quantification (LLOQ) was 1.0ng/mL. The intra and inter-run precisions were less than 6.95 and 7.22%, respectively. The method was successfully applied to determine the plasma concentrations of andrographolide in Chinese volunteers.
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