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  • Title: A set of piezoelectric biosensors using cholinesterases.
    Author: Teller C, Halámek J, Makower A, Scheller FW.
    Journal: Methods Mol Biol; 2009; 504():3-22. PubMed ID: 19159087.
    Abstract:
    Piezoelectric sensors have become a versatile tool in biosensorics to study protein-protein and protein-small molecule interactions. Here we present theoretical background on piezoelectric sensors and instructions, how to modify their surface with various recognition elements for cholinesterases. These recognition elements comprise an organophosphate (paraoxon), a cocaine derivative (BZE-DADOO), and a tricyclic, aromatic compound (propidium). Additionally, a guide to the kinetic evaluation of the obtained binding curves is given in this chapter.
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