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  • Title: [RP-hPLC determination of flavonoids in several flowers].
    Author: Niu YF, Shao Y, Zhao XH, Wen HX, Tao YD.
    Journal: Zhongguo Zhong Yao Za Zhi; 2008 Sep; 33(18):2102-4. PubMed ID: 19160795.
    Abstract:
    OBJECTIVE: To develvp a RP-HPLC method for the determination of flavonoids in fifteen kinds of flowers such as Iris lacteal pall, prunus persica and rosa chinensis. METHOD: The contents of quercetin, kaempferol and isorhamntin in fifteen kinds of flowers were extracted with methanol. The analysis was performed on a Kromasil C18 column (4.6 mm x250 mm, 5 microm) with methanol-0.1% phosphoric acid (50:50) as mobile phase. RESULT: The quercetin, kaempferol and isorhamntin were separated well, and the result shows that the content of quercetin in the Iris lactea Pall was the highest (1.536%), the contene of kaempferol in Persica persice was the highest (0.572%), and the content of isorhamntin in chrysamthemum morifolium was up to 0.290%. CONCLUSION: The contents of flavonoids in these flowers were by determined RP-HPLC for the first time and the method can be used for quantitative determination of flavonoids in the flowers.
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