These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: SNP genotyping using the Sequenom MassARRAY iPLEX platform.
    Author: Gabriel S, Ziaugra L, Tabbaa D.
    Journal: Curr Protoc Hum Genet; 2009 Jan; Chapter 2():Unit 2.12. PubMed ID: 19170031.
    Abstract:
    The method for SNP genotyping described in this unit is based on the commercially available Sequenom MassARRAY platform. The assay consists of an initial locus-specific PCR reaction, followed by single base extension using mass-modified dideoxynucleotide terminators of an oligonucleotide primer which anneals immediately upstream of the polymorphic site of interest. Using MALDI-TOF mass spectrometry, the distinct mass of the extended primer identifies the SNP allele.
    [Abstract] [Full Text] [Related] [New Search]