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  • Title: [Expression of PML-RAR alpha fusion transcripts detection by using RQ-PCR].
    Author: Han LX, Lin J, Qian J, Wang YL, Qian Z, Yang XF, Sheng XJ, Yao DM.
    Journal: Zhonghua Xue Ye Xue Za Zhi; 2008 Nov; 29(11):753-6. PubMed ID: 19176013.
    Abstract:
    OBJECTIVE: To establish the real time quantitative PCR (RQ-PCR) assay according to 'Europe Against Cancer' (EAC) program and analyze the results of detection and quantification of different PML-RAR alpha transcript isoforms in patients with acute promyelocytic leukemia (APL). METHODS: Three RQ-PCR systems were performed to detect the most frequent PML-RAR alpha transcripts (L-form, S-form and V-form) in 30 APL patients and the RQ-PCR end-point products were identified by electrophoresis. RESULTS: S-form RQ-PCR system could amplify positive signals of three isoforms in all of 30 cases, and V-form RQ-PCR system could do so in both L-form and V-form positive cases, however, L-form RQ-PCR system could only do so in L-form-positive cases. Electrophoresis and sequencing of end-point products amplified by S-form RQ-PCR system revealed three bands in each of L-form (621 bp, 477 bp and 218 bp) and V-form (567 bp, 423 bp and 218 bp) positive patients samples. CONCLUSIONS: RQ-PCR, sensitive and reliable, can be used for monitoring the minimal residual disease in APL patients, however, its results should be interpreted carefully if it is used for detection of PML-RAR alpha fusion transcripts prospectively.
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